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Photobiocatalytic oxyfunctionalization in continuous flow

Selin Kara, Photo by Melissa B. Kirkeby Yildirim
Selin Kara, Photo by Melissa B. Kirkeby Yildirim

By combining nature’s own reactions, Associate Professor Selin Kara aims to develop a fully green and sustainable production process for chemicals.


With a total of DKK 6.2 million (EUR 0.8 mill.) of prestigious Sapere Aude funding from the Independent Research Fund Denmark (DFF), Associate Professor Selin Kara, chemical engineer and expert in biocatalysis and bioprocesses, is launching a new research project which could eventually secure completely environmentally friendly production of chemicals.

Selin Kara will use entirely natural enzymatic reactions to synthesize chemicals in a continuous flow: Imagine small tubes (around a few millilitres) into which you put one product in one end, and get a high-value product out from the other.

“The idea is to link photo-catalysis with bio-catalysis in miniaturized, tubular bioreactors operated with continuous flow. The system will be multi-phasic where the photobiocatalyst – an enzyme found in normal chestnut mushrooms – will be in a solid form and the reaction media will be liquid. Due to the low solubility of the substrate, also a second liquid phase is needed for bioprocess development. Overall, I will deal with a multi-parameter challenge,” says Selin Kara.

Building blocks for the industry

The photocatalytic part of the system is constantly exposed to light, which is necessary in the process of creating the desired core substrate. The enzyme converts that core substrate (now an intermediate) into the final target product, a pharmaceutical compound.

“The goal is photobiocatalytic synthesis of various active pharmaceutical ingredients which can be used as building blocks to create other chemical substances,” says the associate professor.

The final goal of the project, called PHOTOX- f, Photobiocatalytic oxyfunctionalization in continuous flow, is highly productive photobiocatalysis in continuous flow, which may have a major impact on the pharmaceutical industry.

“If we can utilize bio-catalysis and photo- catalysis together to develop very productive enzymatic reactions, hopefully we’ll make the enzymatic synthesis more attractive in future chemicals production. This will be a huge achievement for the chemicals industry as a whole, as chemical synthesis today is very polluting,” says Selin Kara.

Mini models first

There are, however, some major challenges to be overcome first, which is also why the project is starting with miniature bioreactors:

“The entire system needs refining to the smallest detail to ensure the optimum flow and conditions. The photo- and bio-catalysts have to be perfectly balanced to work together effectively, and that’s why we’re starting by focusing on the spatial spread between the two types of catalysis,” she says.

Sapere Aude grants are awarded by the Independent Research Fund Denmark to talented early career researchers to enable them to develop and strengthen their own research ideas and establish themselves as research directors with the foundation’s financial assistance.

In 2019, the Independent Research Fund Denmark awarded Sapere Aude grants to 35 researchers on the basis of 346 applications. Of these, 11 recipients are from Aarhus University.

A completely environmentally friendly production of chemicals. That’s the goal for Associate Professor Selin Kara, Aarhus University, who’ve received a prestigious Sapere Aude grant of DKK 6.2 million from the Independent Research Fund Denmark. In the project, Selin Kara will merge photo-catalysis with bio-catalysis in miniaturized, tubular bioreactors in the hope of continuously synthesizing chemicals using only natural enzymatic reactions.

PROJECT FACTS

Project title

PHOTOX-f, Photobiocatalytic oxyfunctionalization in continuous flow

Financial framework

DKK 6.2 million, Independent Research Foundation Denmark

About the research section

Contact

Selin Kara

Associate professor